| 货号 | MAB15692-SP |
| 别名 | alpha 2-thiol Proteinase Inhibitor; BDK; KNG1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Kininogen Heavy Chain in direct ELISAs and Western blots. It reacts with HK, Kininogen, and the heavy chain, but not with the light chain alone. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E, S, SA, SN, rhFetuin A, rhFetuin B, rhHPRG, recombinant mouse Kininogen, or rhKininostatin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3827 (Human); 16644 (Mouse); 24903 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Aberrant processing of plasma vitronectin and high-molecular-weight kininogen precedes the onset of preeclampsia. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Kininogen Gln19-Ser644 (Ile581Thr) Accession # P01042 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The HMW form is synthesized as a 644 amino acid (aa) precursor with a signal peptide (aa 1‑18). The mature chain (aa 19‑644) is further processed into the heavy (aa 19‑380) and the light (aa 390‑644) chains. The active peptide bradykinin (aa 381‑389) is released, which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases. The light chain consists of a His-rich domain, which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (427 aa) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (aa 402‑427). The LMW form is not involved in blood clotting. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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