| 货号 | MAB22851-SP |
| 别名 | CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog | 全称 | Checkpoint Kinase 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects endogenous mouse Chk2 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11200 (Human); 50883 (Mouse); 114212 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Chk2 Met1-Ala234 Accession # Q9Z265 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Checkpointkinase2 (Chk2) is a critical protein kinase involved in the cellular response to DNA damage. Chk2 is a target of the ATM protein kinase, and can phoshorylate multiple proteins, including Cdc25A and p53, to activate the G1/S cell cycle checkpoint. Patients with Li-Fraumeni syndrome, a cancer-prone familial disorder, may have a mutation in the chk2orp53 genes, which suggests that Chk2 also functions as a tumor suppressor. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Chk2 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Chk2 Monoclonal Antibody (Catalog # MAB22851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Chk2 at approximately 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
