Human BRCA2 MAb (Clone 234403) (25 UG)【科瑞奥博】

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Human BRCA2 MAb (Clone 234403) (25 UG)

货号： MAB2476-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2476-SP
别名	BRCA1/BRCA2-containing complex, subunit 2; BRCC2; breast and ovarian cancer susceptibility gene, early onset; breast cancer 2 tumor suppressor; breast cancer 2, early onset; breast cancer susceptibility protein BRCA2; breast cancer type 2 susceptibility protein; BROVCA2; FACD; FAD; FAD1; FANCB; FANCD; FANCD1Fanconi anemia, complementation group D1; Fanconi anemia group D1 protein; GLM3; PNCA2
全称	Breast Cancer 2
反应种属	Human
应用	Western Blot(0.2 µg/mL) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects endogenous human BRCA2.
使用方法	Western Blot: 0.2 µg/mL Immunohistochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	675 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines Authors: K Ishiguro, YL Zhu, ZP Lin, PG Penketh, K Shyam, R Zhu, RP Baumann, AC Sartorelli, TJ Rutherford, ES Ratner J Transl Sci, 2016;2(2):117-124. Species: Human Sample Type: Cell Lysates Application: WB RAS promotes tumorigenesis through genomic instability induced by imbalanced expression of Aurora-A and BRCA2 in midbody during cytokinesis. Authors: Yang G, Mercado-Uribe I, Multani A, Sen S, Shih I, Wong K, Gershenson D, Liu J Int J Cancer, 2013;133(2):275-85. Species: Human Sample Type: Cell Lysates Application: WB Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2. Authors: Yang G, Chang B, Yang F Clin. Cancer Res., 2010;16(12):3171-81. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human BRCA2 Met1-Thr200 Accession # P51587
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	BRCA2 is a nuclear protein that acts as a tumor suppressor. Germline mutation of BRCA2 accounts for many cases of familial breast and ovarian cancer. At the cellular level, BRCA2 is involved in homologous recombination (HR), a form of double-strand break repair. BRCA2 interacts with the Rad51 recombinase, another protein critical for HR, to preserve chromosomal integrity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Checkpoint Nucleotide Excision Repair/Transcription Coupled Repair

参考图片

Detection of Human BRCA2 by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.