Human CD300a/LMIR1 MAb (Clone 232612) (25 UG)【科瑞奥博】

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Human CD300a/LMIR1 MAb (Clone 232612) (25 UG)

货号： MAB2640-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2640-SP
别名	CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human CD300a/LMIR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human LMIR2 (CMRF-35A) is observed and no cross-reactivity with recombinant human LMIR3, 4, 5, or 6 is observed.
使用方法	Western Blot: Human granulocytes under non-reducing conditions only Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	11314 (Human); 217303 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Y3 rat myeloid cell line transfected with human CD300a/LMIR1
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CD300a, also known as LMIR1 (in rodents), CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300a consists of a 163 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2). Alternative splicing may generate additional isoforms that either lack the Ig-like domain or contain only the cytoplasmic domain. Within the ECD, human CD300a shares 40% and 43% aa sequence identity with mouse and rat LMIR1, respectively. In human, CD300a is expressed on peripheral blood eosinophils, mast cells, neutrophils, plasmacytoid dendritic cells, and various T cell subsets (3‑7). Antibody crosslinking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Crosslinking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (5, 10‑12). CD300a crosslinking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 11, 12).
运输条件	Blue Ice
存放说明	4℃
参考文献	Clark, G.J. et al. (2009) Trends Immunol. 30:209. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148. Munitz, A. et al. (2006) Blood 107:1996. Bachelet, I. et al. (2005) J. Immunol. 175:7989. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253. Ju, X. et al. (2008) Blood 112:1184. Clark, G.J. et al. (2007) J. Leukoc. Biol. 82:1126. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223. Bachelet, I. et al. (2008) J. Immunol. 180:6064. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.

参考图片

Detection of Human CD300a/LMIR1 by Western Blot. Western blot shows lysates of human granulocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human CD300a/LMIR1 Monoclonal Antibody (Catalog # MAB2640) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD300a/LMIR1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.