| 货号 | MAB2209-SP |
| 别名 | ATC2; atrial natriuteric peptide-converting enzyme; Corin; corin, serine peptidase; EC 3.4.21; EC 3.4.21.-; heart specific serine proteinase; Heart-specific serine proteinase ATC2; MGC119742; pro-ANP-convertase; Pro-ANP-Converting Enzyme; PRSC; serine protease; Transmembrane protease serine 10 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human Corin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10699 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Corin Ser67-Asn1042 Accession # Q9Y5Q5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Corin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Corin at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Corin in HK2 Human Cell Line. Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
