Human HTRA2/Omi MAb (Clone 229926) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human HTRA2/Omi MAb (Clone 229926) (25 UG)

货号： MAB1458-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1458-SP
别名	High temperature requirement protein A2; HtrA serine peptidase 2; HtrA2; HtrA-like serine protease; Omi; OMIOmi stress-regulated endoprotease; PARK13EC 3.4.21.108; PRSS25; PRSS25serine, 25; Serine protease 25; serine protease HTRA2, mitochondrial; Serine proteinase OMI
全称	High Temperature Requirement Protein-2
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects the mitochondria-processed form of human HTRA2/Omi.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	27429 (Human); 64704 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways. Authors: Haudek SB, Taffet GE, Schneider MD, Mann DL J. Clin. Invest., 2007;117(9):2692-701. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins. Authors: Setoguchi K, Otera H, Mihara K EMBO J., 2006;25(24):5635-47. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human HTRA2/Omi Ala134-Glu458 Accession # O43464
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	HTRA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HTRA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HTRA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC₅₀ values for this effect are typically between 0.2 and 1.5 μM. HTRA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site.
运输条件	Blue Ice
存放说明	4℃
参考文献	Suzuki, Y. et al. (2001) Mol. Cell. 8:613. van Loo, G. et al. (2002) Cell Death & Diff. 9:20. Hedge, R. et al. (2001) J. Biol. Chem. 277:432. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445. Martins, L. et al. (2002) J. Biol. Chem. 277:439. Silke, J., and A. Verhagen (2002) Cell Death & Diff.9:362. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.

参考图片

Detection of Human HTRA2/Omi by Western Blot. Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line, MCF‑7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA2/Omi at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.