Human/Mouse/Rat JNK1 MAb (Clone 228611) (25 UG)【科瑞奥博】

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Human/Mouse/Rat JNK1 MAb (Clone 228611) (25 UG)

货号： MAB1776-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1776-SP
别名	c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; MAPK8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8; PRKM8JNK; protein kinase JNK1; SAPK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1
全称	C-Jun N-terminal Kinase 1
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human, mouse, and rat JNK1, expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. Does not detect recombinant JNK2 or JNK3.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5599 (Human); 26420 (Mouse); 116554 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumor necrosis factor-alpha-elicited stimulation of gamma-secretase is mediated by c-Jun N-terminal kinase-dependent phosphorylation of presenilin and nicastrin. Authors: Kuo LH, Hu MK, Hsu WM, Tung YT, Wang BJ, Tsai WW, Yen CT, Liao YF Mol. Biol. Cell, 2008;19(10):4201-12. Species: Hamster Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human JNK1 isoform 2 Met1-Gln384 Accession # NP_002741
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases C-type Lectin Receptors GDNF Family IL-1 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Neurotrophin/Trk Family NOD-like Receptors and the Inflammasome PDGF Family Positive Regulators of the Jak/STAT Pathway TNF Superfamily Regulators Toll-Like Receptors Wnt Intracellular Signaling

参考图片

Detection of Human, Mouse, and Rat JNK1 by Western Blot.

Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK1 were detected at approximately 54 kDa and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Detection of human JNK1 by Western Blot.

Western blot shows recombinant human JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.