货号 | MAB1857-SP |
别名 | 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin | 全称 | Neutrophil Gelatinase-associated Lipocalin |
反应种属 | Mouse |
应用 | Western Blot(0.5 µg/mL) |
目标/特异性 | Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin‑2. |
使用方法 | Western Blot: 0.5 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3934 (Human); 16819 (Mouse); 170496 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2 aa 21-200 Accession # P11672 |
内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Lipocalin-2, also known as Neutrophil Gelatin-Associated Lipocalin (NGAL) and Siderocalin, mediates the innate immune response to bacterial infection by sequestering iron-laden siderophores. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Mouse Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin‑2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |