货号 | MAB2140-SP |
别名 | Atia; Protein slit-like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; vasorin |
反应种属 | Human |
应用 | Western Blot(1 µg/mL) |
目标/特异性 | Detects human Vasorin in direct ELISAs and Western blots. |
使用方法 | Western Blot: 2 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 114990 (Human); 246154 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Vasorin aa 24-575 Accession # Q6EMK4 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Vasorin is a type I membrane protein that is predominantly expressed in vascular smooth muscle cells in a developmentally regulated pattern. Expression is down-regulated during vessel repair after arterial injury. Vasorin directly binds to transforming growth factor (TGF)-beta and attenuates TGF-beta signaling in vitro (1). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human Vasorin/SLIT‑like 2 by Western Blot. Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT‑like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT‑like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |