Human CD25/IL-2 R alpha MAb (Clone 22722) (25 UG)【科瑞奥博】

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Human CD25/IL-2 R alpha MAb (Clone 22722) (25 UG)

货号： MAB223-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB223-SP
别名	CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
全称	Interleukin 2 Receptor alpha
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma, rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha, rhIL-6 R, rhIL‑7 R, rhIL-9 R, and rhIL-10 R is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 0.5-1.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
来源	Monoclonal Mouse IgG₁ Clone # 22722
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cytokine modulation of defensive rage behavior in the cat: role of GABAA and interleukin-2 receptors in the medial hypothalamus. Authors: Bhatt S, Zalcman S, Hassanain M, Siegel A Neuroscience, 2005;133(1):17-28. Species: Feline Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Lymphoblastic Leukemia Acute Myeloid Leukemia B Cell Activation B Cell Markers Cancer Biomarkers Common gamma Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Tfh Cell Differentiation and Function Group 1 ILCs Group 2 ILCs Human CD Marker Products ICC / IHC Images: CD25/IL-2 R alpha Interactive Pathway: IL-2 Signaling and its Primary Biological Effects in Different Immune Cell Types Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Lymphoma NCR+ILC3 NCR-ILC3 Neuroinflammation Preeclampsia Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) T Cell Cytokine Signaling

参考图片

CD25/IL‑2 R alpha in Human PBMCs. CD25/IL‑2 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB223). The ND₅₀ is typically 0.5‑1.0 µg/mL.