| 货号 | MAB1408-SP |
| 别名 | CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Cystatin B in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cystatins A and S, recombinant mouse Cystatin B, rhFetuin A, and rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed and approximately 5% to 10% cross-reactivity with rhCystatins C, D, E/M, SA, SN, and rhFetuin B is observed. |
| 使用方法 | Western Blot: 2 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Cystatin B (0.53 µg/mL, Catalog # 1408-PI) inhibition of Papain (0.1 µg/mL) cleavage of the fluorogenic peptide substrate Z-FR-AMC (100 µM, Catalog # ES009). The Neutralization Dose (ND50) is typically 5.4 µg/mL. |
| 来源 | Monoclonal Mouse IgG2B Clone # 225228 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1476 (Human); 13014 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Cystatin B aa Met2-Phe98 Accession # P04080 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cystatin B by Western Blot. Western blot shows lysates of human colon tissue, SK‑BR‑3 human breast cancer cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cystatin B Monoclonal Antibody (Catalog # MAB1408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cystatin B at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Neutralization of Cystatin B Activity by Human Cystatin B Antibody. Papain (0.1 µg/mL) activity is measured in the presence of Recombinant Human Cystatin B (0.53 µg/mL, Catalog # 1408-PI) that has been preincubated with increasing concentrations of Human Cystatin B Monoclonal Antibody (Catalog # MAB1408). The ND50 is typically 5.4 µg/mL. |
