| 货号 | MAB1494-SP |
| 别名 | BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B | 全称 | Neurotrophic Tyrosine Kinase Receptor B |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects mouse TrkB in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75‑100% cross-reactivity with recombinant human (rh) TrkB is observed and no cross-reactivity with rhTrkA, recombinant rat TrkA, or recombinant mouse TrkC is observed. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4915 (Human); 18212 (Mouse); 25054 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TrkB Cys32-His429 Accession # P15209 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5‑6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse TrkB by Western Blot. Western blot shows lysate of mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TrkB Monoclonal Antibody (Catalog # MAB1494) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
