Mouse LYVE-1 MAb (Clone 223322) (25 UG)【科瑞奥博】

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Mouse LYVE-1 MAb (Clone 223322) (25 UG)

货号： MAB2125-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2125-SP
别名	cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1XLKD1; XLKD1
全称	Lymphatic Vessel Endothelial Hyaluronan Receptor 1
反应种属	Mouse
应用	Western Blot(2 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.
使用方法	Western Blot: 2 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.1 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Mouse LYVE-1 (Catalog # 2125-LY) coated at 5 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding. Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10894 (Human); 114332 (Mouse); 293186 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration Authors: Tsun-Ho Lo J Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen A micro-sterile inflammation array as an adjuvant for influenza vaccines. Authors: Wang J, Shah D, Chen X, Anderson R, Wu M Nat Commun, 2014;5(0):4447. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes. Authors: Reichardt, Peter, Patzak, Irene, Jones, Kristian, Etemire, Eloho, Gunzer, Matthias, Hogg, Nancy EMBO J, 2013;32(6):829-43. Species: Mouse Sample Type: Whole Tissue Application: IF FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus. Authors: Zhi L, Kim P, Thompson BD, Pitsillides C, Bankovich AJ, Yun SH, Lin CP, Cyster JG, Wu MX J. Immunol., 2011;187(5):2244-51. Species: Mouse Sample Type: In Vivo Application: Intravital imaging TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM J. Clin. Invest., 2009;119(10):2954-64. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth. Authors: Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7137-42. Species: Mouse Sample Type: Whole Cells Application: Flow CXCR4-gp120-IIIB interactions induce caspase-mediated apoptosis of prostate cancer cells and inhibit tumor growth. Authors: Singh S, Bond VC, Powell M, Singh UP, Bumpers HL, Grizzle WE, Lillard JW Mol. Cancer Ther., 2009;8(1):178-84. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells. Authors: Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG Nat. Immunol., 2009;10(1):58-65. Species: Mouse Sample Type: In Vivo Application: In vivo B lymphocytes exit lymph nodes through cortical lymphatic sinusoids by a mechanism independent of sphingosine-1-phosphate-mediated chemotaxis. Authors: Sinha RK, Park C, Hwang IY, Davis MD, Kehrl JH Immunity, 2009;30(3):434-46. Species: Mouse Sample Type: In Vivo Application: In vivo CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells. Authors: Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, Sakabe J, Yoshiki R, Tamamura H, Fujii N, Inaba K, Tokura Y Am. J. Pathol., 2007;171(4):1249-57. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Authors: Pham TH, Okada T, Matloubian M, Lo CG, Cyster JG Immunity, 2007;28(1):122-33. Species: Mouse Sample Type: Whole Cells Application: Flow Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells. Authors: Phan TG, Grigorova I, Okada T, Cyster JG Nat. Immunol., 2007;8(9):992-1000. Species: Mouse Sample Type: Whole Tissue Application: IHC Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor. Authors: Backhed F, Crawford PA, O&amp;apos;Donnell D, Gordon JI Proc. Natl. Acad. Sci. U.S.A., 2007;104(2):606-11. Species: Mouse Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF/3 mouse pro-B cell line transfected with mouse LYVE-1 Ala24-Thr234 Accession # Q8BHC0
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397. Banerji, S. et al. (1999) J. Cell Biol. 144:789. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420. Carreira, C.M. et al. (2001) Cancer Research 61:8079. Jackson, D.J. et al. (2001) Trends Immunol. 22:317. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

参考图片

Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE‑1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).