| 货号 | MAB4091-SP |
| 别名 | Cytokine P40; HP40; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40 | 全称 | Interleukin 9 |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-9. |
| 使用方法 | Neutralization: Measured by its ability to neutralize IL‑9-induced proliferation in the TS1 mouse helper T cell line. Moreau, F. J. et al. (1988) Nature 336:690. The Neutralization Dose (ND50) is typically 0.5-2.0 ng/mL in the presence of 0.05 ng/mL Recombinant Mouse IL‑9. |
| 来源 | Monoclonal Rat IgG2B Clone # 222622 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3578 (Human); 16198 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9 Gln19-Pro144 Accession # P15247.1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. Mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% amino acid sequence homology. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. Recombinant Mouse IL‑9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑9 Monoclonal Antibody (Catalog # MAB4091). The ND50 is typically 0.5-2.0 ng/mL. |
