货号 | MAB1566-SP |
别名 | GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; hGH-N; Somatotropin |
反应种属 | Rat |
应用 | Western Blot(2 µg/mL) |
目标/特异性 | Detects rat Growth Hormone in direct ELISAs and Western blots. Does not cross‑react with recombinant human Growth Hormone. |
使用方法 | Western Blot: 2 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 2688 (Human) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant rat Growth Hormone Phe27-Phe216 Accession # P01244 |
生物活性 | Rat |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Growth Hormone, also known as somatotropin, is synthesized primarily inthe anterior pituitary. It is a pleiotropic cytokine that exerts itsbiological actions by binding to the transmembrane GH receptor which ispresent in many cell types. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Rat Growth Hormone by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Growth Hormone Monoclonal Antibody (Catalog # MAB1566) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Growth Hormone at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |