Human Podocalyxin MAb (Clone 222328) (25 UG)【科瑞奥博】

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Human Podocalyxin MAb (Clone 222328) (25 UG)

货号： MAB1658-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1658-SP
别名	GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin-like protein 1; podocalyxin-like; PODXL
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5420 (Human); 27205 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome. Authors: Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G PLoS ONE, 2013;8(10):e75945. Species: Human Sample Type: Whole Tissue Application: IHC - paraffin Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury. Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P Stem Cells, 2012;30(8):1714-25. Species: Human Sample Type: Whole Cells Application: Flow Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders. Authors: Lasagni L, Ballerini L, Angelotti ML Stem Cells, 2010;28(9):1674-85. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Regeneration of glomerular podocytes by human renal progenitors. Authors: Ronconi E, Sagrinati C, Angelotti ML J. Am. Soc. Nephrol., 2009;20(2):322-32. Species: Human Sample Type: Whole Cells Application: cell sorting
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Podocalyxin Ser23-Arg427 Accession # AAB61574
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Li, J. et al. (2001) DNA Seq. 12:407. Hara, T. et al. (1999) Immunity 11:567.

参考图片

Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.