| 货号 | MAB1700-SP |
| 别名 | GATA binding protein 6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) GATA-1 or rhGATA-2. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2627 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human GATA-6 Met1-Ala449 Accession # CAA64997 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | GATA-6 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-6 is expressed in visceral and parietal endoderm and other tissues. It interacts with several additional proteins to regulate gene expression in heart, lung, gut and gonads (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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GATA‑6 in HCT‑116 Human Cell Line. GATA‑6 was detected in immersion fixed HCT‑116 human colorectal carcinoma cell line using Mouse Anti-Human GATA‑6 Monoclonal Antibody (Catalog # MAB1700) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
GATA‑6 in HT‑29 Human Cell Line. GATA‑6 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Mouse Anti-Human GATA‑6 Monoclonal Antibody (Catalog # MAB1700) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
