Human TNF RII/TNFRSF1B MAb (Clone 22221) (25 UG)【科瑞奥博】

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Human TNF RII/TNFRSF1B MAb (Clone 22221) (25 UG)

货号： MAB226-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB226-SP
别名	CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
全称	Tumor Necrosis Factor Receptor II
反应种属	Human
应用	Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human TNF RII/TNFRSF1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTNF RI, or recombinant mouse TNF RII is observed. Neutralizes the biological effect of rhTNF RII but not rhTNF RI.
使用方法	Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG_2A Clone # 22221
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7133 (Human); 21938 (Mouse); 156767 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy. Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y PLoS ONE, 2015;10(4):e0122212. Species: Human Sample Type: Whole Tissue Application: IHC FOXO3a regulates oxygen-responsive expression of tumor necrosis factor receptor 2 in human dermal microvascular endothelial cells. Authors: Ding B, Kirkiles-Smith NC, Pober JS J. Biol. Chem., 2009;284(29):19331-9. Species: Human Sample Type: Cell Lysates Application: WB Cutting edge: TNFR-shedding by CD4+CD25+ regulatory T cells inhibits the induction of inflammatory mediators. Authors: van Mierlo GJ, Scherer HU, Hameetman M, Morgan ME, Flierman R, Huizinga TW, Toes RE J. Immunol., 2008;180(5):2747-51. Species: Human Sample Type: Whole Cells Application: Flow Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression. Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF Atherosclerosis, 2007;192(1):25-32. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Neut Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1). Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X Exp. Cell Res., 2004;298(2):560-73. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human TNF RII Leu23-Asp257 Accession # P20333
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with highaffinity and is expressed primarily in cells of the immune system.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell Structure and Signaling Molecules Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy Endothelial Cell Markers Human CD Marker Products ICC / IHC Images: TNF RII/TNFRSF1B Neuroinflammation Obesity Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members Regulatory T Cells (Tregs) T Cell Cytokine Signaling TNF Superfamily TNF Superfamily Receptors

参考图片

TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B inhibits Recombinant Human TNF‑ alpha (Catalog # 210-TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/
TNFRSF1B (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/
TNFRSF1B Monoclonal Antibody (Catalog # MAB226). The ND₅₀ is typically 0.5-1.5 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF RII/TNFRSF1B in Human PBMCs. TNF RII/TNFRSF1B was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.