货号 | MAB2371-SP |
别名 | FGF-5; fibroblast growth factor 5; HBGF-5; Heparin-binding growth factor 5; Smag-82 | 全称 | Fibroblast Growth Factor 5 |
反应种属 | Human |
应用 | Immunohistochemistry(8-25 µg/mL) |
目标/特异性 | Detects human FGF-5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-3, -4, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, -23, recombinant mouse FGF-8b, -8c, -15, or -23 is observed. |
使用方法 | Immunohistochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 2250 (Human) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human FGF-5 long isoform Glu23-Gly268 (Lys238Asn, Pro245Ser) Accession # Q8NF90 |
内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | FGF-5 is a member of the FGF family of mitogenic peptides. FGF 5 cDNA encodes a 267 amino acid residue protein with a putative 22 amino acid residue signal peptide. In vivo, FGF-5 is suggested to play important roles in both embryology and neurobiology. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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FGF‑5 in Human Breast Cancer Tissue. FGF‑5 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human FGF‑5 Monoclonal Antibody (Catalog # MAB2371) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
FGF‑5 in Human Breast Cancer Tissue. FGF‑5 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human FGF‑5 Monoclonal Antibody (Catalog # MAB2371) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |