Human/Mouse/Rat ERK1/ERK2 MAb (Clone 216703) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse/Rat ERK1/ERK2 MAb (Clone 216703) (25 UG)

货号： MAB1576-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1576-SP
别名	ERK1/ERK2
全称	Extracellular Signal-regulated Kinase 1/2
反应种属	Human/Mouse/Rat
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human, mouse, and rat ERK1/ERK2 in Western blots.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells J Biol Chem, 2016;291(19):10277-92. Species: Human Sample Type: Cell Lysates Application: WB The carotenoid lutein enhances matrix metalloproteinase-9 production and phagocytosis through intracellular ROS generation and ERK1/2, p38 MAPK, and RARbeta activation in murine macrophages. Authors: Lo H, Chen C, Yang C, Wu P, Tsou C, Chiang K, Wu W J Leukoc Biol, 2013;93(5):723-35. Species: Mouse Sample Type: Cell Lysates Application: WB Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways. Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G PLoS ONE, 2012;7(9):e45257. Species: Human Sample Type: Cell Lysates Application: WB Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C J. Mol. Cell. Cardiol., 2010;48(6):1225-35. Species: Human Sample Type: Cell Lysates Application: WB The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages. Authors: Lane AE, Tan JT, Hawkins CL Biochem. J., 2010;430(1):161-9. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human ERK1 Met1-Pro379 Accession # P27361
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family C-type Lectin Receptors GDNF Family ICC / IHC Images: ERK1/ERK2 IL-1 Family IL-17 Family Interactive Pathway: IL-1 Family Signaling Pathways Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Mini-review: The ERK Signal Transduction Pathway Natural Killer T (NKT) Cells Neurotrophin/Trk Family PDGF Family Positive Regulators of the Jak/STAT Pathway ROS and Stress Resistance TNF Superfamily Regulators VEGF Family

参考图片

Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

ERK1/ERK2 in Human Brain. ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.