| 货号 | MAB1680-SP |
| 别名 | CCN family member 4; CCN4; CCN4WISP1tc; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1 | 全称 | Wnt-1 Induced Secreted Protein 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse WISP-1/CCN4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 10-30% cross-reactivity with recombinant human (rh) WISP-1 but no cross-reactivity with rhCTGF or rmNOV. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8840 (Human); 22402 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse WISP-1/CCN4 Leu21-Asn367 Accession # O54775 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1- 6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84%, and 85% aa identical to rat, canine, and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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