| 货号 | MAB1348-SP |
| 别名 | calcineurin subunit B type 1; CALNB1; CNA2; Cnb1; MCIP1; PP2B beta 1; PPP3R1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; type I (19kDa) |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat Calcineurin B. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5534 (Human); 19058 (Mouse); 29748 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Calcineurin B Met1-Val170 Accession # P63098 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Background Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6). References: 1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859. 2. Zeng H. et al. (2001) Cell 107:617. 3. Okamura, H. et al. (2000) Molecular Cell 6:539. 4. Liu, J. et al. (1992) Biochemistry 31:3896. 5. Molkentin, J.D. (2000) Circulation Research 87:731. 6. Sanquer, S.A. et al. (2004) Transplantation 77:854. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, TS1 mouse helper T cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Calcineurin B Monoclonal Antibody (Catalog # MAB1348) followed by HRP-conjugated Anti‑Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
