Zebrafish VEGF MAb (Clone 211615) (25 UG)【科瑞奥博】

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Zebrafish VEGF MAb (Clone 211615) (25 UG)

货号： MAB1247-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1247-SP
别名	MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
全称	Vascular Endothelial Growth Factor
反应种属	Zebrafish
应用	Western Blot(1 µg/mL)
目标/特异性	Detects zebrafish VEGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) VEGF₁₂₁,₁₆₅,₂₀₆, rhVEGF-B₁₈₆, rhVEGF-C, rhVEGF‑D, recombinant mouse (rm) VEGF₁₁₅, rmVEGF₁₆₅, rmVEGF-B₁₈₆, rmVEGF‑D, or recombinant rat VEGF₁₆₄ is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize VEGF₁₆₅-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.2-0.5 µg/mL in the presence of 100 ng/mL Recombinant Zebraship VEGF₁₆₅.
来源	Monoclonal Mouse IgG_2B Clone # 211615
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis. Authors: Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA J. Biol. Chem., 2008;283(23):16084-92. Species: Zebrafish Sample Type: Tissue Homogenates Application: WB Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology. Authors: Vigneswaran N, Sacks P, Gilcrease M, Zacharias W J. Oral Pathol. Med., 2005;34(2):77-86. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant zebrafish VEGF₁₆₅ Ala24-Arg188 Accession # O73682.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Zebrafish
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF₁₆₅ shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF₁₆₅, respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish (FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as upregulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1‑7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14. Laing, D. and R. Ge (1998) GenBank Accession # AAC14713. Laio, W. et al. (1997) Development 124:381. Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470. Thurston, G. (2002) J. Anat. 200:575. Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

参考图片

Cell Proliferation Induced by VEGF₁₆₅ and Neutralization by Zebrafish VEGF Antibody.

Recombinant Zebrafish VEGF₁₆₅(Catalog #1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅ (100 ng/mL) is neutrallized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND₅₀ is typically 0.2‑0.5 µg/mL.