| 货号 | MAB1144-SP |
| 别名 | delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; PREF1; Pref-1; protein delta homolog 1; secredeltin; ZOG | 全称 | Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human Pref‑1/DLK‑1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8788 (Human); 13386 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Pref-1 long isoform Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions) Accession # P80370 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Pref-1 (Preadipocyte factor 1; also DLK-1 and FA1) is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins. It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages. Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383). The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25‑31 kDa fragments that show no activity. There are multiple potential splice variants. One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1‑52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383. Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Pref‑1/DLK1/FA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). | |
Pref‑1/DLK1/FA1 in HepG2 Human Cell Line. Pref‑1/DLK1/FA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB1144) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
