| 货号 | MAB111-SP |
| 别名 | BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2 | 全称 | Bone Morphogenetic Protein 2 |
| 反应种属 | Zebrafish |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects zebrafish BMP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human BMP-2, -3, -3B, -4, -5, -6, -7, -8, or recombinant zebrafish BMP-4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize BMP‑2a-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 1 µg/mL Recombinant Zebrafish BMP‑2a and 2 µg/mL heparin. |
| 来源 | Monoclonal Mouse IgG1 Clone # 208301 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 650 (Human); 12156 (Mouse); 29373 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant zebrafish BMP‑2 Gln272-Arg386 Accession # AAI63048 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Zebrafish |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | BMP-2 is one of at least 20 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF‑ beta ) superfamily (1, 2). BMPs were originally identified as protein regulators of cartilage and bone formation. However, they are also involved in embryogenesis and morphogenesis of various tissues and organs. BMPs regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse, and rat BMP-2 share 100% amino acid (aa) sequence identity, while mature human BMP-2 and zebrafish BMP-2 share 85% aa identity. Zebrafish have another homolog of BMP-2 (also called BMP-2a) called BMP-2b, which is 88% identical to BMP-2 in the mature region and corresponds to the swirlmutant. The combined expression pattern of zfBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates (3). Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 115 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero‑ogliomeric complexes of type I and type II serine/threonine kinase receptors (4). In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both type I BMP receptor’s can bind BMP-2 with high affinity in the absence of BMP receptor type II. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Alkaline Phosphatase Production Induced by BMP‑2a and Neutralization by Zebrafish BMP‑2 Antibody. Recombinant Zebrafish BMP‑2a (Catalog # 111‑BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Zebrafish BMP‑2a (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Zebrafish BMP‑2 Monoclonal Antibody (Catalog # MAB111). The ND50 is typically 5‑25 µg/mL in the presence of heparin (2 µg/mL). |
