Human JAM-C MAb (Clone 208206) (25 UG)【科瑞奥博】

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Human JAM-C MAb (Clone 208206) (25 UG)

货号： MAB1189-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1189-SP
别名	CD323; JAM-2; JAM3; JAMC; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C
全称	Junctional Adhesion Molecule C
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human JAM-C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human JAM-A, recombinant mouse (rm) JAM-A, or rmJAM-C is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize JAM‑C-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND₅₀) is typically 0.01-0.05 µg/mL in the presence of 0.2 µg/mL Recombinant Human JAM‑B Fc Chimera.
来源	Monoclonal Mouse IgG₁ Clone # 208206
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	83700 (Human); 83964 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Constitutive and functionally relevant expression of JAM-C on platelets. Authors: Erpenbeck L, Rubant S, Hardt K, Santoso S, Boehncke WH, Schon MP, Ludwig RJ Thromb. Haemost., 2010;103(4):857-9. Species: Human Sample Type: Cell Lysates Application: WB Possible involvement of gap junctions in the barrier function of tight junctions of brain and lung endothelial cells. Authors: Nagasawa K, Chiba H, Fujita H, Kojima T, Saito T, Endo T, Sawada N J. Cell. Physiol., 2006;208(1):123-32. Species: Porcine Sample Type: Cell Lysates Application: WB Antibody blockade of junctional adhesion molecule-A in rabbit corneal endothelial tight junctions produces corneal swelling. Authors: Mandell KJ, Holley GP, Parkos CA, Edelhauser HF Invest. Ophthalmol. Vis. Sci., 2006;47(6):2408-16. Species: Rabbit Sample Type: Whole Tissue Application: IHC Fresh
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human JAM-C Val32-Asn241 (Ala149Pro) Accession # Q9BX67
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 46 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3, 4). Human JAM-C shares 86% aa sequence identity with its mouse homologue. It also shares approximately 36% and 32% aa sequence homology with human JAM-B and JAM-A, respectively (3‑5). Human JAM-C shows widespread tissue expression and the highest levels are found in the placenta, brain, kidney and heart. JAM-C is expressed on endothelial cells of high endothelial venules in human tonsil. It is also expressed on platelets, T-cells and NK cells (3‑5). Unlike other JAM family members, JAM-C forms only weak homotypic interactions. JAM-C binds to JAM-B to facilitate the interactions between JAM-B and the integrin alpha4beta1 (6). This heterotypic interaction between leukocyte JAM-C and endothelial JAM-B may play a role in regulating leukocyte transmigration (5). On platelets, JAM-C is a counter-receptor for the leukocyte integrin Mac-1(CD11b/CD18) (7). JAM-C has also been identified as a strong candidate gene for hypoplastic left heart syndrome (8). The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B. Under this system, JAM-C refers to the protein encoded by the gene localized to human chromosome 11.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chavakis, T. et al. (2003) Thromb. Haemost. 89:13. Aurand-Lions, M. et al. (2001) Blood 98:3699. Arrate, M.P. et al. (2001) J. Biol. Chem. 276:45826. Liang, T. et al. (2002) J. Immunol. 168:1618. Johnson-Leger, C. et al. (2002) Blood 100:25793. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589. Santoso, S. et al. (2002) J. Exp. Med. 196:679. Phillips, H.M. et al. (2002) Genomics 79:475.

参考图片

Cell Adhesion Mediated by JAM‑C and Neutralization by Human JAM‑C Antibody. Recombinant Human JAM‑B/VE-JAM Fc Chimera, immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G‑102‑C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM‑B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human JAM-C Monoclonal Antibody (Catalog # MAB1189). The ND₅₀ is typically 0.01‑0.05 µg/mL.