TGF-beta 3 MAb (Clone 20724) (25 UG)【科瑞奥博】

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TGF-beta 3 MAb (Clone 20724) (25 UG)

货号： MAB243-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB243-SP
别名	ARVD; FLJ16571; TGFB3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3
全称	Transforming Growth Factor beta 3
应用	Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects TGF-beta 3 from multiple species in direct ELISAs and Western blots. In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF‑ beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross‑reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. Neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize TGF‑ beta 3 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 0.1 ng/mL Recombinant Human TGF‑ beta 3 and 7.5 ng/mL Recombinant Mouse IL‑4.
来源	Monoclonal Mouse IgG₁ Clone # 20724
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7043 (Human); 21809 (Mouse); 25717 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ Mol. Biol. Cell, 2011;22(10):1686-98. Species: Canine Sample Type: Whole Cells Application: Neut Differential versican isoforms and aggrecan expression in the chicken embryo aorta. Authors: Arciniegas E, Neves CY, Candelle D, Parada D Anat Rec A Discov Mol Cell Evol Biol, 2004;279(1):592-600. Species: Chicken Sample Type: Whole Tissue Application: IHC Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	Spodoptera frugiperda, Sf 21 (baculovirus) derived recombinant human TGF-beta 3 Ala301-Ser412 (Tyr340Phe) Accession # P10600
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF‑ beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Lin, J.S. et al. (2006) Reproduction 132:179. Hinck, A.P. et al. (1996) Biochemistry 35:8517. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261. Derynck, R. et al. (1988) EMBO J. 7:3737. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

参考图片

TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody. Recombinant Human TGF‑ beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB243). The ND₅₀ is typically 0.1-0.3 µg/mL.

TGF‑ beta 3 in Human Breast Cancer Tissue. TGF‑ beta 3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB243) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.