| 货号 | MAB1569-SP |
| 别名 | alpha 2-thiol Proteinase Inhibitor; BDK; KNG1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Kininogen in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3827 (Human); 16644 (Mouse); 24903 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Kininostatin Lys438-Ser531 Accession # P01042 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The HMW form is synthesized as a 644 amino acid (aa) precursor with a signal peptide (aa 1‑18). The mature chain (aa 19‑644) is further processed into the heavy (aa 19‑380) and the light (aa 390‑644) chains. The active peptide bradykinin (aa 381‑389) is released, which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases. The light chain consists of a His-rich domain, which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (427 aa) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (aa 402‑427). The LMW form is not involved in blood clotting. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Kininogen in Human Brain. Kininogen was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Kininogen Monoclonal Antibody (Catalog # MAB1569) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
