| 货号 | MAB1202-SP |
| 别名 | CST5; CSTD; cystatin 5; cystatin D; Cystatin-5; cystatin-D; cysteine-proteinase inhibitor; MGC71922 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, E/M, S, SA, SN, rhKininogen, rhFetuins A, B, or rhHPRG is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Cystatin D (1.3 µg/mL, Catalog # 1202-PI) inhibition of Papain (0.1 mg/mL) cleavage of the fluorogenic peptide substrate Z-FR-AMC (100 mM, Catalog # ES009). The Neutralization Dose (ND50) is typically 2.8 µg/mL. |
| 来源 | Monoclonal Mouse IgG2A Clone # 206006 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1473 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Cystatin D Arg28-Val142 Accession # P28325 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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