Human/Primate CXCL1/GRO alpha MAb (Clone 20326) (25 UG)【科瑞奥博】

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Human/Primate CXCL1/GRO alpha MAb (Clone 20326) (25 UG)

货号： MAB275-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB275-SP
别名	CINC-1; GRO alpha; KC; MGSA-alpha
反应种属	Human
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In Western blots, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) CXCL2/GRO beta and rhCXCL3/GRO gamma and no cross-reactivity with recombinant rat (rr)CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhMIP-1 alpha, recombinant mouse (rm)MIP-1 alpha, rmMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 3-15 µg/mL in the presence of 10 ng/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1.
来源	Monoclonal Mouse IgG_2B Clone # 20326
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2919 (Human); 14825 (Mouse); 81503 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke. Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T Blood, 2012;120(18):3793-802. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO J. Biol. Chem., 2011;286(4):2636-47. Species: Human Sample Type: Whole Cells Application: ICC The expression and prognostic impact of CXC-chemokines in stage II and III colorectal cancer epithelial and stromal tissue. Authors: Oladipo O, Conlon S, OGrady A Br. J. Cancer, 2011;104(3):480-7. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Cell Culture Supernates Application: Neut Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells. Authors: Hagiwara H, Mitsumata M, Yamane T Circulation, 1998;98(23):2584-90. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. It remains to be seen if a unique GRO receptor(s) also exist. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors ICC / IHC Images: CXCL1/GRO alpha/KC/CINC-1 Inflammatory Mediators Macrophage Activation Markers

参考图片

Detection of CXCL1/GRO alpha /KC/CINC‑1 in Human PBMCs Treated with LPS and Monensin by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) treated with 1 μg/mL LPS for 24 hours and
3 μM monensin for 2 hours was stained with Mouse Anti-Human/Primate CXCL1/GRO alpha /
KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody. Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GRO alpha /KC/CINC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275). The ND₅₀ is typically 3-15 µg/mL.