Human Glut1 MAb (Clone 202915) (25 UG)【科瑞奥博】

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Human Glut1 MAb (Clone 202915) (25 UG)

货号： MAB1418-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1418-SP
别名	DYT17; DYT18; DYT18DYT17; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GLUT1DS; GLUT1GLUT; HepG2 glucose transporter; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; solute carrier family 2, facilitated glucose transporter member 1
全称	Glucose Transporter Type 1
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6513 (Human); 20525 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells. Authors: Basu S, Hubbard B, Shevach E J Leukoc Biol, 2015;97(2):279-83. Species: Human Sample Type: Whole Cells Application: Flow Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection. Authors: Palmer C, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge D, Maisa A, Hearps A, Lewin S, Landay A, Jaworowski A, McCune J, Crowe S AIDS, 2014;28(3):297-309. Species: Human Sample Type: cells Application: Flow The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis. Authors: Chan O, Burke J, Gao D, Fish E J Biol Chem, 2012;287(35):29406-16. Species: Human Sample Type: Whole Cells Application: Flow Entry of human T-cell leukemia virus type 1 is augmented by heparin sulfate proteoglycans bearing short heparin-like structures. Authors: Tanaka A, Jinno-Oue A, Shimizu N J. Virol., 2012;86(6):2959-69. Species: Human Sample Type: Whole Cells Application: Flow DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells. Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B J. Virol., 2009;83(21):10908-21. Species: Human Sample Type: Whole Cells Application: Flow Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates. Authors: Luty WH, Rodeberg D, Parness J, Vyas YM J. Immunol., 2007;179(2):819-29. Species: Human Sample Type: Whole Cells Application: ICC Role of FDG-PET as a biological marker for predicting the hypoxic status of tongue cancer. Authors: Han M, Lee H, Cho K, Kim J, Roh J, Choi S, Nam S, Kim S Head Neck, 0;34(10):1395-402. Species: Human Sample Type: tissue Application: IHC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human Glut1 Met1-Val492 Accession # AAA52571
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8⁺ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.
运输条件	Blue Ice
存放说明	4℃
参考文献	Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713. Meuckler, M. et al. 1985, Science 229:941. Jones, K.S. et al. 2006, J. Virol. 8291. Takenouchi, N. et al. 2007, J. Virol. 1506. Kinet, S. et al. 2007, Retrovirology 4:31.

参考图片

Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Glut1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.