| 货号 | MAB1415-SP |
| 别名 | FLJ90380; GLUT-3; GLUT3Glucose transporter type 3, brain; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2, facilitated glucose transporter member 3 | 全称 | Glucose Transporter Type 3 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(0.25 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6515 (Human); 20527 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human Glut3 Accession # P11169 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Glut3 was stained with Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. | |
Glut3 in SH‑SY5Y Human Cell Line. Glut3 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
