| 货号 | MAB1044-SP |
| 别名 | AP endonuclease 1; APE; APE1; APE-1; APEAPEX nuclease (multifunctional DNA repair enzyme); APENAP endonuclease class I; APEX deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEX1; Apurinic-apyrimidinic endonuclease 1; APXAP lyase; DNA-(apurinic or apyrimidinic site) lyase; EC 3.1; EC 4.2.99.18; HAP1apurinic/apyrimidinic (abasic) endonuclease; multifunctional DNA repair enzyme; protein REF-1; redox factor 1; Redox factor-1; REF1 apurinic/apyrimidinic exonuclease; REF-1 | 全称 | Apurinic/Apyrimidinic Endonuclease |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat APE in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 328 (Human); 305605 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human APE Pro2-Leu318 Accession # P27695 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF‑ kappa B, and p53. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat APE by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, Raji human Burkitts lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat APE Monoclonal Antibody (Catalog # MAB1044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
