货号 | MAB1874-SP |
别名 | CMH2; CMPD2; cTnT; TNNT2; TnTC |
反应种属 | Human |
应用 | Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects purified human Troponin T (Cardiac) in direct ELISAs. |
使用方法 | Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 7139 (Human) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Human cardiac Troponin T |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Troponin T in BG01V Human Embryonic Stem Cells. Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. | |
Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |