Human/Mouse Neprilysin Ectodomain MAb (Clone 200103) (25 UG)【科瑞奥博】

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Human/Mouse Neprilysin Ectodomain MAb (Clone 200103) (25 UG)

货号： MAB1126-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1126-SP
别名	Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human and mouse Neprilysin/CD10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ECE-2 or recombinant mouse Kell is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4311 (Human); 17380 (Mouse); 24590 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Polyhydroxycurcuminoids but not curcumin upregulate neprilysin and can be applied to the prevention of Alzheimer's disease Sci Rep, 2016;6(0):29760. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Neprilysin/CD10 Tyr52-Trp750 Accession # Q61391
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Neprilysin is also known as CALLA and CD10. It is a zinc metalloprotease expressed at the cell surface that cleaves a variety of bioactive peptides such as enkephalins, atrial natriuretic peptide and amyloid beta peptide.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Lymphoblastic Leukemia APP Cleavage and Amyloid-beta Degradation B Cell Markers Hematopoietic Progenitor Cell Markers Human CD Marker Products ICC / IHC Images: Neprilysin/CD10 Interactive Pathway: A-beta Uptake & Degradation Leukocyte Receptors/Markers in Tfh Cell Differentiation and Function Lymphoid Lineage Markers Metalloproteases and Regulators Neutrophils Renal Markers Renin-Angiotensin System T Cell Migration/Adhesion

参考图片

Detection of Human and Mouse Neprilysin/CD10 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Mouse Neprilysin (Catalog # 1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.