Human/Mouse/Rat Cytochrome c MAb (Clone 2.7D5) (25 UG)
货号：
MAB899-SP 基本售价：
1467.3 元 规格：
-
产品信息
概述| 货号 | MAB899-SP |
| 别名 | CYCHCS; CYCS; cytochrome c; cytochrome c, somatic; THC4 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Immunoprecipitation(1 µg/106cells)
|
| 目标/特异性 | Detects native human, mouse, and rat Cytochrome c. |
| 使用方法 | Immunoprecipitation: 1 µg/106cells
|
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 54205 (Human) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Pigeon Cytochrome c |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cytochrome c is a critical mitochondrial outermembrane-associated protein in the electron transport chain (1). Reduction and oxidation of an iron molecule(Fe3+ to Fe2+ and back) within its central heme groupallow it to receive an electron from the cytochrome c1 subunit of cytochromereductase and pass it to cytochrome a within the cytochrome oxidase complex (1). Release of cytochrome c from mitochondriaoccurs during initiation of apoptotic pathways, either by mechanisms thatrupture or that do not rupture the mitochondial membrane (2, 3). Once in the cytosol, cytochrome c forms acomplex with procaspase 9, recruiting Apaf-1 (apoptotic protease activatingfactor-1) and dATP to form apoptosomes, which activate caspase and ultimatelydestroy the cell (2, 3). Cytochrome c is highly conserved, with 90% or greater amino acididentity among human, mouse, rat, cow and dog sequences (4). After cytochrome c translation, the initialmethionine is cleaved to form the 104 amino acid mature form. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Zubay, G. L., 1983, “Biochemistry”, pp. 380, Addison-Wesley Pub. Co, Inc.
- Liu, X. et al., 1996, Cell 86:147.
- Li, P. et al., 1997, Cell 91:479.
- Matsubara, H and E. L. Smith, 1963, J. Biol. Chem. 238:2732.
|
参考图片Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immunoprecipitated from lysates (5 x 10 6 cells) of Jurkat human acute T cell leukemia and CTLL‑2 mouse cytotoxic T cell line following incubation with 5 µg Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB899) for 1 hour at 4 °C. Cytochrome c-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Mouse Anti-Human/ Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation. |
