| 货号 | MAB3346-SP |
| 别名 | CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 |
| 反应种属 | Canine |
| 应用 | Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects canine CD34 in Western blots. |
| 使用方法 | Western Blot: McSweeney, P.A. et al. (1998) Blood 91(6):1977. This application was not tested by R&D Systems. Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of monoclonal antibodies that recognize canine CD34. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Canine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CD34 is a sialomucin molecule that is expressed on primitivehematopoietic stem cells and downregulated as they differentiate intomature cells. Although its precise function remains unknown, thepattern of expression of CD34 suggests that it plays a significant rolein early hematopoiesis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL‑1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Monoclonal Antibody (Catalog # MAB3346, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. |
