| 货号 | MAB5835-SP |
| 别名 | EC 2.7.11.1; MGC117401; MGC138280; MGC142070; PCH1; serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1 | 全称 | Vaccinia-related Kinase 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(1-25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human VRK1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 1-25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7443 (Human); 22367 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human VRK1 Met1-Lys396 Accession # Q99986 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Vaccinia-related kinase 1 (VRK1; also serine/threonine-protein kinase VRK1) is a 45 kDa member of the protein kinase superfamily, the CK1 Serine/Threonine protein kinase family, and the VRK subfamily. Human VRK1 is 396 amino acids (aa) in length, and contains one protein kinase domain (aa 37‑317). Human VRK1 shares 91% and 78% aa sequence identity with bovine and mouse VRK1, respectively. VRK1 is widely expressed with high expression in fetal liver, testis and thymus. VRK1 functions as a serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human VRK1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VRK1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
VRK1 in HeLa Human Cell Line. VRK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
VRK1 in Human Testis. VRK1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
