| 货号 | MAB440-SP |
| 别名 | B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Specifically binds mouse CD40/TNFRSF5 expressed on L cells. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunoprecipitation: Heath, A.W. et al. (1994) Eur. J. Immunol. 24:1828. Agonist Activity: Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. The ED50 for this effect is typically 0.03-0.18 μg/mL (1). |
| 来源 | Monoclonal Rat IgG2A Clone # 1C10 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 958 (Human); 21939 (Mouse); 171369 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. B-cell receptor signal strength influences terminal differentiation. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse CD40/TNFRSF5 Extracellular domain |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. The ED50 for this effect is typically 0.03-0.18 μg/mL (1). |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|