| 货号 | MAB1463-SP |
| 别名 | Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) | 全称 | v-Akt Murine Thymoma Viral Oncogene Homolog 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human Akt3 in Western blots. Using Western blots, this antibody does not detect recombinant human Akt1 or Akt2. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10000 (Human); 23797 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Akt3 Met1-Glu479 Accession # Q9Y243 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma ) and RAC-gamma serine/threonine kinase (RAC-PK-gamma ), is a member of the Akt family that also includes Akt1 (PKB alpha ) and Akt2 (PKB beta ). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Akt3 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human Akt3 Monoclonal Antibody (Catalog # MAB1463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Akt3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
