Rat B7-2/CD86 MAb (Clone 199622) (25 UG)【科瑞奥博】

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Rat B7-2/CD86 MAb (Clone 199622) (25 UG)

货号： MAB13401-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB13401-SP
别名	Activation B7-2 antigen; B70; B7-2 antigen; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86
反应种属	Rat
应用	Neutralization
目标/特异性	Detects rat B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat B7-1, recombinant human B7-2, recombinant mouse (rm) B7-2, rmB7-H1, rmB7-H2, rmB7-H3, or rmPD‑L is observed.
使用方法	Neutralization: Measured by its ability to neutralize B7‑2/CD86 and PHA-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. 87:5031. The Neutralization Dose (ND₅₀) is typically 0.3-1.0 µg/mL in the presence of 1 µg/mL Recombinant Rat B7‑2/CD86 Fc Chimera and 10 µg/mL PHA.
来源	Monoclonal Mouse IgG₁ Clone # 199622
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	942 (Human); 12524 (Mouse); 56822 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant rat B7‑2/CD86 Met1-Ile250 Accession # NP_064466
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	B7-2/CD86 and the related B7-1/CD80 are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. B7-2 is highly expressed on activated antigen presenting cells and vascular endothelial cells. It binds to CD28 on T cells to enhance T cell activation. It additionally binds CTLA-4 which inhibits the T cell response and supports regulatory T cell function. The relative participation of B7-1 and B7-2 in T cell co-stimulation can alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Lymphoblastic Leukemia B Cell Activation B Cell Markers Common Macrophage Markers Control of Immune Responses via CD28 and CTLA-4 Co-stimulatory and Co-inhibitory Molecules Co-stimulatory Molecules Associated with Tfh Cells CTLA-4: Hiding in Plain Sight Dendritic Cell Co-stimulatory Molecules Dendritic Cell Development Flow Cytometry & Cell Selection/Detection Human CD Marker Products ICC / IHC Images: B7-2/CD86 Macrophage Activation Markers Mini-review: B7 & CD28 Superfamilies Mini-review: B7 & CD28/CTLA-4 Receptor Families Mini-review: Dendritic Cell Maturation Mini-review: Immune Tolerance Mini-review: Th1/Th2 Multiple Myeloma Myeloid-derived Suppressor Cells (MDSC) Regulatory T Cells (Tregs) T Cell Antigen Recognition Technical Notes: B7 Family Research Tools

参考图片

IL‑2 Secretion Induced by B7‑2/CD86 and Neutralization by Rat B7‑2/CD86 Antibody.

Recombinant Rat B7‑2/CD86 Fc Chimera (Catalog # 1340-B2) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Rat B7‑2/CD86 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat B7‑2/CD86 Monoclonal Antibody (Catalog # MAB13401). The ND₅₀ is typically 0.3‑1.0 µg/mL.