| 货号 | MAB1436-SP |
| 别名 | Igfals | 全称 | IGF-binding Protein Acid Labile Subunit |
| 反应种属 | Mouse |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects mouse Acid Labile Subunit (ALS) in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 16005 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Acid Labile Subunit Thr28-Cys603 Accession # P70389 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Acid Labile Subunit (ALS) is a liver-derived serum protein that forms a ternary complex with IGFBP-3 and either IGF-I or IGF-II. The presence of ALS increases the half life of IGF/IGFBP-3 complexes in the circulation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse ALS by Western Blot. Western blot shows mouse serum. PVDF Membrane was probed with 2 µg/mL of Mouse ALS Monoclonal Antibody (Catalog # MAB1436) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for the ALS/IGFBP-3 complex at approximately 140 kDa (as indicated) and ALS at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. |
