Mouse Rae-1 MAb (Clone 199215) (25 UG)【科瑞奥博】

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Mouse Rae-1 MAb (Clone 199215) (25 UG)

货号： MAB1758-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1758-SP
别名	Rae1; Rae-1
全称	Retinoic Acid Early Transcript 1
反应种属	Mouse
应用	Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse Rae‑1 alpha / beta / gamma. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha, beta and gamma. It does not recognize Rae-1δ or epsilon.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # 139-NK) to immobilized Recombinant Mouse Rae-1 beta Fc Chimera (Catalog # 1198-RA) coated at 1 µg/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding. This antibody also blocks the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha and Rae-1 gamma.
来源	Monoclonal Rat IgG_2A Clone # 199215
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8480 (Human); 19368 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity. Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N Leukemia, 2014;28(6):1316-25. Species: Mouse Sample Type: Whole Cells Application: Flow Ras activation induces expression of Raet1 family NK receptor ligands. J. Immunol., 2012;189(4):1826-34. Species: Mouse Sample Type: Whole Cells Application: Flow Tripeptidyl-peptidase II controls DNA damage responses and in vivo gamma-irradiation resistance of tumors. Authors: Hong X, Lei L, Kunert B, Naredla R, Applequist SE, Grandien A, Glas R Cancer Res., 2007;67(15):7165-74. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Mini-review: NKG2D and its Ligands Natural Killer T (NKT) Cells New Tools for Cancer Research: Cystatin C, Galectin-3, Rae-1 beta NKG2D Receptors and Ligands

参考图片

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae‑1 alpha / beta / gamma Monoclonal Antibody (Catalog # MAB1758, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.