4-Hydroxynonenal MAb (Clone 198960) (25 UG)【科瑞奥博】

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4-Hydroxynonenal MAb (Clone 198960) (25 UG)

货号： MAB3249-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3249-SP
别名	4 HNE; HNE
应用	Western Blot(1 µg/mL) Simple Western(5 µg/mL)
目标/特异性	Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 5 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene Authors: J Seo, NN Singh, EW Ottesen, S Sivanesan, M Shishimoro, RN Singh PLoS ONE, 2016;11(4):e0154390. Species: Mouse Sample Type: tissue lysates Application: WB Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies. Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N Am J Physiol Endocrinol Metab, 2014;306(4):E443-56. Species: Rat Sample Type: tissue homogenate supernatant Application: WB Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease. J Neuroinflammation, 2012;9(0):241. Species: Mouse Sample Type: Tissue Homogenates Application: WB Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress. Diabetes, 2012;61(8):2093-104. Species: Mouse Sample Type: Cell Lysates Application: WB 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARgamma and accelerating ubiquitin-proteasome degradation. Authors: Wang Z, Dou X, Gu D, Shen C, Yao T, Nguyen V, Braunschweig C, Song Z Mol. Cell. Endocrinol., 2011;349(2):222-31. Species: Mouse Sample Type: Tissue Homogenates Application: WB Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress. Authors: Walson KH, Tang M, Glumac A, Alexander H, Manole MD, Ma L, Hsia CJ, Clark RS, Kochanek PM, Kagan VE, Bayr H Crit. Care Med., 2011;39(2):335-43. Species: Rat Sample Type: Cell Lysates Application: WB Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. Authors: Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song WJ, Node K, Taguchi R, Yamagata K, Oike Y J. Biol. Chem., 2011;286(5):3992-4002. Species: Mouse Sample Type: Cell Lysates Application: WB Cell sex determines anoikis resistance in vascular smooth muscle cells. Authors: Straface E, Vona R, Gambardella L, Ascione B, Marino M, Bulzomi P, Canu S, Coinu R, Rosano G, Malorni W, Franconi F FEBS Lett., 2009;583(21):3448-54. Species: Rat Sample Type: Whole Cells Application: ICC Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis. Authors: Margutti P, Matarrese P, Conti F, Colasanti T, Delunardo F, Capozzi A, Garofalo T, Profumo E, Rigano R, Siracusano A, Alessandri C, Salvati B, Valesini G, Malorni W, Sorice M, Ortona E Blood, 2007;111(9):4559-70. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	KLH-coupled 4-Hydroxynonenal
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
运输条件	Blue Ice
存放说明	4℃
参考文献	Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway Pro-oxidant Molecules

参考图片

Detection of 4-Hydroxynonenal by Western Blot.
Western blot shows recombinant E. coliDNAK treated (+) with 4-hydroxynonenal or peroxynitrite and lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with 4-hydroxynonenal. PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human 4‑Hydroxynonenal by Simple Western^TM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at approximately 4-Hydroxynonenal adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.