| 货号 | MAB1244-SP |
| 别名 | ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase | 全称 | Double Minute 2 Protein |
| 反应种属 | Human |
| 应用 | Immunoprecipitation |
| 目标/特异性 | Detects human MDM2/HDM2. |
| 使用方法 | Immunoprecipitation: 2.5 µg/500 µg cell lysate |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4193 (Human); 17246 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human MDM2/HDM2 Asn3-Pro491 Accession # Q00987 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Immunoprecipitation of Human MDM2. U2OS human osteosarcoma cell line was untreated (-) or treated (+) with proteasome inhibitor LLnV for 1 hour. MDM2 was immunoprecipitated from 500 μg of cell lysate following incubation with 2‑3 µg Mouse Anti-Human MDM2 Monoclonal Antibody (Catalog # MAB1244) for 1 hour on ice. MDM2-antibody complexes were absorbed using goat anti-mouse agarose (Sigma). Immunoprecipitated MDM2 was detected by Western blot using 1 µg/mL Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244). View our recommended buffer recipes for immunoprecipitation. |
