Mouse p53 MAb (Clone 197643) (25 UG)
货号：
MAB1746-SP 基本售价：
1467.3 元 规格：
-
产品信息
概述| 货号 | MAB1746-SP |
| 别名 | Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 |
| 反应种属 | Mouse |
| 应用 | Immunoprecipitation |
| 目标/特异性 | Detects mouse p53 in Western blots. |
| 使用方法 | Immunoprecipitation: 2 µg/500 µg cell lysate
|
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 7157 (Human); 22059 (Mouse); 24842 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Early-life nutrition influences thymic growth in male mice that may be related to the regulation of longevity.
Authors: Chen JH, Tarry-Adkins JL, Heppolette CA, Palmer DB, Ozanne SE
Clin. Sci., 2010;118(6):429-38.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB
Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
Authors: Chen JH, Jones RH, Tarry-Adkins J, Smith NH, Ozanne SE
Exp. Cell Res., 2008;314(16):3075-80.
Species: Rat
Sample Type: Cell Lysates
Application: WB |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse p53
Leu28-Phe325
Accession # NP_035770 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Apoptosis Transcription Factors and Regulators
- Cancer Biomarkers
- Cancer Stem Cell Transcription Factors
- Cell Cycle
- Checkpoint
- DNA Damage and Repair
- Epithelial Stem Cell Transcription Factors
- Hematopoietic Stem Cell Transcription Factors and Regulators
- ICC / IHC Images: p53
- Immunoglobulin-Like Domain Transcription Factors
- Mesenchymal Stem Cell Transcription Factors
- Mini-review: DNA Damage Response
- Mini-review: Genomic Instability Syndromes
- Neural Stem Cell Transcription Factors
- p53 Pathway
- p53: More than a Transcription Factor?
- Parkinsons Disease Related Molecules
- Pluripotent Stem Cell Transcription Factors
- TNF Superfamily Regulators
- Transcription Factors and Regulators
- Transcription Factors in the Akt Pathway
|
参考图片Immunoprecipitation of Mouse p53.Balb/3T3 mouse embryonic fibroblast cell line was untreated
(-) or treated (+) with 1 μM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 μg of cell lysate following incubation with 1‑3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). View our recommended buffer recipes for immunoprecipitation. |
