| 货号 | MAB13351-SP |
| 别名 | Cytokine ML-1; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24 | 全称 | Interleukin 17F |
| 反应种属 | Human |
| 应用 | CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL‑17A, B, C, D, or E. |
| 使用方法 | Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 112744 (Human); 257630 (Mouse); 301291 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TH17 cytokines induce human airway smooth muscle cell migration. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-17F Arg31-Gln163 Accession # Q96PD4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑17F in Activated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13351) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. | |
IL‑17F in Human Th17 Cells. IL‑17F was detected in immersion fixed human T helper 17 cells using Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13351) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
