Human Nestin MAb (Clone 196908) (25 UG)【科瑞奥博】

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Human Nestin MAb (Clone 196908) (25 UG)

货号： MAB1259-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1259-SP
别名	FLJ21841; NES; nestin
反应种属	Human
应用	CyTOF-reported,Immunocytochemistry,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human Nestin.
使用方法	Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10763 (Human); 18008 (Mouse); 25491 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype PLoS ONE, 2016;11(5):e0155106. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells. Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M Stem Cells, 2015;33(4):1142-52. Species: Human Sample Type: Whole Cells Application: Flow A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT). Authors: Jeitany M, Pineda J, Liu Q, Porreca R, Hoffschir F, Desmaze C, Silvestre D, Mailliet P, Junier M, Londono-Vallejo A, Segal-Bendirdjian E, Chneiweiss H, Boussin F Int J Cancer, 2015;136(7):1546-58. Species: Human Sample Type: Whole Cells Application: IHC Frozen The evidence of glioblastoma heterogeneity. Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M Sci Rep, 2015;5(0):7979. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma. Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T Int J Oncol, 2014;44(4):1118-30. Species: Human Sample Type: Whole Cells Application: ICC Embryonic stem cell-based modeling of tau pathology in human neurons. Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P Am J Pathol, 2013;182(5):1769-79. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow. Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M PLoS ONE, 2012;7(5):e35639. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Authors: Emdad L, DSouza SL, Kothari HP, Qadeer ZA, Germano IM Stem Cells Dev., 2012;21(3):404-10. Species: Human Sample Type: Whole Cells Application: ICC Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes. Authors: Engelen E, Akinci U, Bryne JC Nat. Genet., 2011;43(6):607-11. Species: Human Sample Type: Whole Cells Application: ICC Expression of the stem cell markers nestin and CD133 on circulating melanoma cells. Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U J. Invest. Dermatol., 2011;131(2):487-94. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Glial cell type-specific subcellular localization of 14-3-3 zeta: an implication for JCV tropism. Authors: Lamba S, Ravichandran V, Major EO Glia, 2009;57(9):971-7. Species: Human Sample Type: Whole Cells Application: ICC Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH FEBS Lett., 2009;583(3):561-7. Species: Human Sample Type: Whole Cells Application: ICC Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling. Authors: Yuki K, Natsume A, Yokoyama H, Kondo Y, Ohno M, Kato T, Chansakul P, Ito M, Kim SU, Wakabayashi T Cancer Lett., 2009;284(1):71-9. Species: Human Sample Type: Whole Cells Application: ICC Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures. Authors: Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V Invest. Ophthalmol. Vis. Sci., 2008;49(12):5325-31. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Authors: Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, Smith A Mol. Cell. Neurosci., 2008;38(2):245-58. Species: Human Sample Type: Whole Cells Application: ICC Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons. Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O Stem Cells, 2008;26(7):1705-12. Species: Human Sample Type: Whole Cells Application: ICC The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6. Authors: Hattermann K, Ludwig A, Gieselmann V, Held-Feindt J, Mentlein R Mol. Cell. Neurosci., 2008;39(1):133-41. Species: Mouse Sample Type: Whole Cells Application: ICC The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress. Authors: Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J Neurosci. Lett., 2007;423(3):225-30. Species: Human Sample Type: Whole Cells Application: ICC Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue. Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD Stem Cells, 2007;25(5):1298-306. Species: Human Sample Type: Whole Cells Application: ICC Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions. Authors: Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T Biochem. Biophys. Res. Commun., 2007;361(3):586-92. Species: Human Sample Type: Whole Cells Application: ICC Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro. Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C Exp. Hematol., 2007;35(7):1119-31. Species: Human Sample Type: Whole Cells Application: ICC Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH FEBS Lett., 2006;580(25):5869-74. Species: Human Sample Type: Whole Cells Application: ICC Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions. Authors: Shin S, Mitalipova M, Noggle S, Tibbitts D, Venable A, Rao R, Stice SL Stem Cells, 2006;24(1):125-38. Species: Human Sample Type: Whole Cells Application: ICC Impaired proliferation and migration in human Miller-Dieker neural precursors. Authors: Sheen VL, Ferland RJ, Harney M, Hill RS, Neal J, Banham AH, Brown P, Chenn A, Corbo J, Hecht J, Folkerth R, Walsh CA Ann. Neurol., 2006;60(1):137-44. Species: Human Sample Type: Cell Lysates Application: WB Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages. Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N Br. J. Haematol., 2006;135(5):703-14. Species: Human Sample Type: Whole Cells Application: Flow Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells. Authors: Gerecht-Nir S, Cohen S, Ziskind A, Itskovitz-Eldor J Biotechnol. Bioeng., 2004;88(3):313-20. Species: Human Sample Type: Whole Cells Application: ICC Differentiation of human embryonic stem cells into insulin-producing clusters. Authors: Segev H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J Stem Cells, 2004;22(3):265-74. Species: Human Sample Type: Whole Cells Application: ICC High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK Brain Res. Dev. Brain Res., 2004;152(2):159-69. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human Nestin
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310. Lendahl, U. et al. (1990) Cell 60:585. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144. Tohyama, T. et. al. (1992) Lab. Invest. 66:303. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720. Frederiksen, K. et al. (1988) Neuron 1:439. Cattaneo, E. et al. (1990) Nature 347:762. Reynolds, B.A. and S. Weiss (1992) Science 255:1707. Rietze, R.L. et al. (2001) Nature 412:736. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383. Zulewski, H. et al. (2001) Diabetes 50:521. Lumelsky, N. et al. (2001) Science 292:1389. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670. Shih, C.C. et al. (2001) Blood 98:2412.

参考图片

Nestin in Human Neural Progenitor Cells. Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.