Human/Mouse/Rat beta-Catenin MAb (Clone 196624) (25 UG)【科瑞奥博】

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Human/Mouse/Rat beta-Catenin MAb (Clone 196624) (25 UG)

货号： MAB13292-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB13292-SP
别名	beta 1 (88kD); beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923
反应种属	Human/Mouse/Rat
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human, mouse, and rat beta -Catenin in Western blots.
使用方法	Western Blot: 2 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1499 (Human); 12387 (Mouse); 84353 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human beta -Catenin Ala2-Leu781 Accession # P35222
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Cancer Stem Cell Transcription Factors Cell to Cell Adhesion Disassembly During EMT Early Endodermal Lineage Markers Epithelial Cell Polarity ICC / IHC Images: beta-Catenin Mesenchymal Cells and EMT Neural Stem Cell Markers Notch Pathway Primitive Endoderm Markers Stem Cell Transcription Factor Regulators Transcription Factors and Regulators Transcriptional Co-Activators Wnt Family Wnt Flips a Dual Switch to Activate Adult Neurogenesis Wnt Intracellular Signaling

参考图片

Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat beta ‑Catenin Monoclonal Antibody (Catalog # MAB13292, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0101B).

Detection of Human/Mouse/Rat beta -Catenin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB13292) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.