Human NKp46/NCR1 MAb (Clone 195314) (25 UG)【科瑞奥博】

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Human NKp46/NCR1 MAb (Clone 195314) (25 UG)

货号： MAB1850-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1850-SP
简介	Clone 195314 was used by HLDA to establish CD designation
别名	CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
反应种属	Human
应用	Western Blot,Flow Cytometry,Agonist Activity,CyTOF-reported,Immunocytochemistry
目标/特异性	Detects human NKp46/NCR1 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Agonist Activity: Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is typically 0.1‑0.3 μg/mL. Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9437 (Human); 17086 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome J Immunol, 2016;197(5):1621-30. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection. Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z Immunity, 2016;45(1):131-44. Species: Human Sample Type: Whole Tissue Application: IHC Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients. Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A PLoS ONE, 2015;10(7):e0133363. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1). Authors: Durrenberger PF, Ettorre A, Kamel F J Neuroinflammation, 2012;9(0):1. Species: Human Sample Type: Whole Cells Application: ICC Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients. Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F PLoS ONE, 2012;7(11):e49135. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human. Authors: Yu J, Mitsui T, Wei M, Mao H, Butchar JP, Shah MV, Zhang J, Mishra A, Alvarez-Breckenridge C, Liu X, Liu S, Yokohama A, Trotta R, Marcucci G, Benson DM, Loughran TP, Tridandapani S, Caligiuri MA J. Clin. Invest., 2011;121(4):1456-70. Species: Human Sample Type: Whole Cells Application: Functional Assay An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation. Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A J. Immunol., 2008;181(3):2227-37. Species: Human Sample Type: Whole Cells Application: Functional Assay Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F Blood, 2007;111(3):1309-17. Species: Human Sample Type: Whole Cells Application: Neut A novel binding assay to assess specificity of monoclonal antibodies. Authors: Warren HS, Warren</LastName><ForeNam HS</Initia, Bettadapura J J. Immunol. Methods, 2005;305(1):33-8. Species: Human Sample Type: recombinant NKp46 coupled to Dynal beads Application: Epitope Mapping
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse T cell hybridoma transfected with human NKp46/NCR1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is typically 0.1‑0.3 μg/mL.
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Moretta, L. and A. Moretta (2004) EMBO J. 23:255. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. Pende, D. et al. (1999) J. Exp. Med. 190:1505. Arnon, T. et al. (2004) Blood 103:664. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680. Mandelboim, O. et al. (2001) Nature 409:1055. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.

参考图片

NKp46/NCR1 in NK‑92 Human Cell Line. NKp46/NCR1 was detected in immersion fixed NK‑92 human natural killer lymphoma cell line using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.